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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. In a clinical microbiology laboratory, quality control testing of Mueller-Hinton agar used for disk diffusion susceptibility testing involves checking the zone sizes for E. coli ATCC 25922, S. aureus ATCC 25923, and
P. aeruginosa ATCC 27853. What is the primary purpose of including P. aeruginosa ATCC 27853 in this QC?

A) To ensure appropriate concentration of calcium and magnesium ions affecting aminoglycoside activity
B) To verify the performance of cell wall active agents like penicillin
C) To assess the performance of agents primarily active against Gram-positive organisms
D) To confirm the correct pH of the agar medium

2. The gut microbiome has been implicated in the pathogenesis of autoimmune diseases. Dysbiosis, a disruption in the composition and function of the gut microbiota, can lead to increased intestinal permeability and the translocation of microbial products that trigger inflammatory responses. Which of the following bacterial components is a potent activator of the innate immune system and has been linked to the development of autoimmunity?

A) Lipoteichoic acid (LTA)
B) Lipopolysaccharide (LPS)
C) Flagellin
D) Peptidoglycan

3. A 6-year-old child presents with a pharyngitis characterized by a grayish-white pseudomembrane covering the tonsils and posterior pharynx. Gram stain of the membrane reveals Gram-positive bacilli. Culture on Loeffler's medium shows growth of metachromatic granules. The MOST significant virulence factor associated with this organism's pathogenicity is:

A) Streptolysin O
B) Diphtheria toxin
C) Protein A
D) Hyaluronidase

4. A clinical microbiology laboratory is investigating a cluster of bloodstream infections caused by Staphylococcus aureus. To differentiate between community-associated (CA-MRSA) and healthcare- associated (HA-MRSA) strains, they may look for the presence of:

A) Specific staphylococcal cassette chromosome mec (SCCmec) types, such as SCCmec IV or V inCA- MRSA.
B) Resistance to non-beta-lactam antibiotics, which is more common in CA-MRSA.
C) The mecA gene, which is common to both CA-MRSA and HA-MRSA.
D) Panton-Valentine leukocidin (PVL) toxin genes, which are exclusively found in HA-MRSA.

5. A microbiology laboratory is performing antimicrobial susceptibility testing using Etest strips. The MIC value is determined by:

A) The diameter of the zone of inhibition around the strip.
B) The lowest concentration at which the elliptical zone of inhibition intersects the strip.
C) The highest concentration that allows visible bacterial growth within the zone of inhibition.
D) The presence or absence of growth at a single critical concentration.

Solutions: